75 research outputs found
Different neuroinflammatory profile in amyotrophic lateral sclerosis and frontotemporal dementia is linked to the clinical phase
Objective To investigate the role of neuroinflammation in asymptomatic and symptomatic amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD) mutation carriers. Methods The neuroinflammatory markers chitotriosidase 1 (CHIT1), YKL-40 and glial fibrillary acidic protein (GFAP) were measured in cerebrospinal fluid (CSF) and blood samples from asymptomatic and symptomatic ALS/FTD mutation carriers, sporadic cases and controls by ELISA. Results CSF levels of CHIT1, YKL-40 and GFAP were unaffected in asymptomatic mutation carriers (n=16). CHIT1 and YKL-40 were increased in gALS (p<0.001, n=65) whereas GFAP was not affected. Patients with ALS carrying a CHIT1 polymorphism had lower CHIT1 concentrations in CSF (-80%) whereas this polymorphism had no influence on disease severity. In gFTD (n=23), increased YKL-40 and GFAP were observed (p<0.05), whereas CHIT1 was nearly not affected. The same profile as in gALS and gFTD was observed in sALS (n=64/70) and sFTD (n=20/26). CSF and blood concentrations correlated moderately (CHIT1, r=0.51) to weak (YKL-40, r=0.30, GFAP, r=0.39). Blood concentrations of these three markers were not significantly altered in any of the groups except CHIT1 in gALS of the Ulm cohort (p<0.05). Conclusion Our data indicate that neuroinflammation is linked to the symptomatic phase of ALS/FTD and shows a similar pattern in sporadic and genetic cases. ALS and FTD are characterised by a different neuroinflammatory profile, which might be one driver of the diverse presentations of the ALS/FTD syndrome
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Optimization and Design of the Minimal Architecture Zinc-Bromine Battery Using Insight from a Levelized Cost of Storage Model
This work demonstrates how a levelized cost of storage (LCOS) model can be used to optimize the performance of the minimal architecture zinc bromine battery (MA-ZBB). Cycling data is collected at charge times ranging from 4 to 48 hours and capacities ranging from 320 to 4000 mAh using scaled-up versions of the MA-ZBB. An LCOS model for the entire MA-ZBB system is proposed and used to demonstrate how the energy efficiency/discharge energy trade-off within the system can be exploited to minimize LCOS. The present, unoptimized cell is shown to approach an LCOS of 0.02 kWh−1. At all purchase prices, greater than 60% of the LCOS comes from the capital cost, where the main contributors are the carbon foam electrode and zinc bromine electrolyte in the cell (both accounting for 20% of the total capital cost). In addition, two case studies are conducted which show how the LCOS model can be used to determine the optimal electrode spacing (0.4 cm) and electrolyte concentration (1.0 M) in the cell. Finally, a comparison with existing technologies is conducted, indicating the system-level cost of the MA-ZBB is competitive with lithium-ion, lead-acid, vanadium redox flow, and zinc bromine redox flow batteries
Cellular expression of human centromere protein C demonstrates a cyclic behavior with highest abundance in the G1 phase.
Centromere proteins are localized within the centromere-kinetochore complex, which can be proven by means of immunofluorescence microscopy and immunoelectron microscopy. In consequence, their putative functions seem to be related exclusively to mitosis, namely to the interaction of the chromosomal kinetochores with spindle microtubules. However, electron microscopy using immune sera enriched with specific antibodies against human centromere protein C (CENP-C) showed that it occurs not only in mitosis but during the whole cell cycle. Therefore, we investigated the cell cycle-specific expression of CENP-C systematically on protein and mRNA levels applying HeLa cells synchronized in all cell cycle phases. Immunoblotting confirmed protein expression during the whole cell cycle and revealed an increase of CENP-C from the S phase through the G2 phase and mitosis to highest abundance in the G1 phase. Since this was rather surprising, we verified it by quantifying phase-specific mRNA levels of CENP-C, paralleled by the amplification of suitable internal standards, using the polymerase chain reaction. The results were in excellent agreement with abundant protein amounts and confirmed the cyclic behavior of CENP-C during the cell cycle. In consequence, we postulate that in addition to its role in mitosis, CENP-C has a further role in the G1 phase that may be related to cell cycle control
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